Tikuyesetsanso zoyeserera za PCR, bwanji zotsatira zake nthawi zonse zimakhala zoyipa kwambiri kuposa ena?

Tikuyesetsanso zoyeserera za PCR, bwanji zotsatira zake nthawi zonse zimakhala zoyipa kwambiri kuposa ena?

Mu labotale, yang'anani pa PCR ya anthu ena, magwiridwe antchito ndiokwera, electrophoresis amavula mwangwiro, ndipo yang'anani pawo, ovuta kuchita kwa nthawi yayitali, kumva kuti zomwe achitezo ndi zabwino kwambiri, zotsatira za mayeso abodza …… vutolo?

Ndikukhulupirira kuti anzathu ambiri adakumana ndi mavuto osiyanasiyana. Tikafika pakhoma pazoyeserera za PCR, titha kusanthula mosamala zomwe zimachitika, koma nthawi zambiri timanyalanyaza zofunikira pazogwiritsa ntchito poyesa.

Zotsatira zanu za PCR ndizolakwika, zogula PCR zitha kukhala chifukwa chofunikira. Kumbali imodzi, kuipitsa kwazinthu zogwiritsa ntchito kapena kuyambitsa zoletsa kuyambitsa kuyipitsa kwamayesero; Kumbali inayi, kusankha kosagwiritsidwa ntchito koyenera kumapangitsanso zotsatira zoyeserera kuvutika.

Vutoli limabweranso. Pali mitundu yambiri yamagetsi ya PCR, ndiye mungasankhe bwanji zoyenera kuti kuyesaku kuyende bwino? Kodi ndi mavuto ati omwe ayenera kulipidwa? Osadandaula, leroI kuphatikiza ndi zaka zambiri zokumana nazo, kusanja zovuta zomwe PCR imagwiritsa ntchito posankha mavuto, ndikuyembekeza kukuthandizani.

 

Q1: Chifukwa chiyani zogwiritsa ntchito za PCR nthawi zambiri zimapangidwa ndi zinthu za PP?

Huida: Chifukwa zida za PCR nthawi zambiri zimalumikizana mwachindunji ndi ma reagents kapena zitsanzo, zida za polypropylene (PP) zimakhala zopanda umunthu, sizitsatira ma biomoleculars, ndipo zimakhala ndi kulolerana kwamankhwala komanso kulolerana kutentha (zitha kusinthidwa pa 121 ° C, amathanso kupirira kutentha kwakanthawi kozungulira).

 

Q2: Ndingasankhe bwanji pakati pamitundu yosiyanasiyana yamachubu / mbale za PCR?

HUIDA: Cholinga cha kusankha: sankhani zinthu zoyenera kutengera zoyeserera. Kukula kwakukulu kwa chubu la PCR kumakwaniritsa zofunikira za PCR. Pachifukwa ichi, tikulimbikitsidwa kuti tisankhe chubu lotsika koyamba. Chifukwa chubu / mbale yotsika yama voliyumu imakhala ndi malo ang'onoang'ono pamutu, madutsidwe akutentha amasintha ndipo madzi amatulutsa madzi. Nthawi yomweyo, ndikofunikira kupewa kupewa kuwonjezera zochulukirapo kapena zochepa kwambiri. Kuchulukitsitsa kumatha kubweretsa kuchepa kwa matenthedwe otentha, kutayika ndi kuipitsidwa kwapakati, pomwe zochepa kwambiri zitha kubweretsa kutaya kwamadzi.

Mafotokozedwe ndi kuchuluka kwa machubu omwe amachita:

Chitubu chimodzi / chophatikizira: 0.5ml, 0.2ml, 0.15ml

Chidebe cha 96: 0.2ml, 0.15ml

Mbale wa chitsime cha 384: 0.04ml

 

Q3: Zitsanzo zambiri zikakhala zazing'ono, ndidzasankha chitoliro chimodzi kapena chitoliro chophatikizika, koma bwanji ena ali ndi chivundikiro chofewa pomwe ena ali ndi chivundikiro chazotulutsa?

Huida: Pamene nyemba zocheperako ndizochepa, chubu limodzi kapena chubu chophatikizira chimasankhidwa. Komabe, voliyumu ikakhala yayikulu, chubu limodzi limakhala lalikulu, ndipo voliyumu imatha kufikira 0,5 mL. Ndipo chivundikiro chosanja ndi chophimba chotchinga chilichonse chili ndi mwayi, zitha kutengera zidziwitso izi.

Chitubu chimodzi: 0.2ml ndi 0.5ml; Mungasankhe kuchuluka kwazomwe mungasinthe.

Kulumikiza chubu: 0.2ml kapena 0.15ml ndiyotheka; Machubu 8 kapena 12 amakhala wamba.

Lathyathyathya chivundikiro: angapereke zolondola kuwala chizindikiro HIV kwa qPCR; Zolemba zosavuta.

Chophimba chophimba: kulumikizana ndi chivundikiro chotentha cha chida cha PCR kuti muchepetse kukonzanso kwa chubu chomwe chimayambitsa kukakamizidwa; Komabe, zidzakhudza kufalikira kwa chizindikiro cha fluorescence ndipo sizingagwiritsidwe ntchito poyesa qPCR.

 

Q4: Chifukwa chiyani ma mbale ena a PCR amakhala ndi masiketi pomwe ena alibe?

Huida: M'malo mwake, siketi ya mbale ya PCR idapangidwa kuti izolowere momwe ingagwiritsire ntchito zokha, kupereka chithandizo chokhazikika komanso kupirira kwamphamvu kwa chida, komanso kukhazikika kwapakati pa kupopera.

Ma mbale a PCR amagawika nthawi zambiri kukhala opanda hemline, theka-hemline komanso ma hemline athunthu.

Popanda Mbale: Oyenera makina ambiri a PCR kapena makina a qPCR, koma osangogwiritsa ntchito zokha. Kukhazikika sikuli kwakukulu pakupopera mapaipi, chifukwa chake kuyenera kugwiritsidwa ntchito ndikuthandizira mbale.

Gawo lakumapeto kwa siketi: limatha kusinthidwa kuti lizilemba kapena kugwiritsa ntchito bar code, ndikugwiritsa ntchito zodziwikiratu, ndipo lili ndi bata labwino.

Mbale yansalu yathunthu yam'mphepete: yoyenera kwambiri ntchito zoyeserera zokha, itha kusinthidwa kuti izigwiritsa ntchito mtundu wa bar. Ili ndi mphamvu zamagetsi, itha kugwiritsidwa ntchito popanga chida cha PCR, ndipo imakhala yolimba popanga mapaipi.

 

Q5 :Kwa mbale za PCR, chifukwa chiyani ngodya ndi zolemba sizofanana?

Huida: Izo zimatengera mphamvu ya ngodya kudula ndi chodetsa.

Kudula pakona: Kusankha kwa kudula kwa ngodya ya PCR mbale zimadalira zofunikira za chida choyenera kuti chikhale chosavuta.

Kuzindikiritsa: Zolemba za Alphanumeric pamapepala a PCR zimathandizira kuzindikira Wells payekha komanso malo oyeserera. Nthawi zambiri kwa embossed mtundu digito Logo kapena cholembedwa logo. Pazinthu zina zamagetsi, mbale zosindikizidwa ndikusindikizidwa zimasindikizidwa bwino.

 

Q6: Chifukwa chiyani ma mbale ena a PCR amakhala otukukika pomwe ena amakhala mosalala? Chabwino ndi chiyani?

Huida: Zomwe zikugwirizana ndizabwino. Pali mitundu iwiri yama mbale wamba a PCR: malire a pore ndi malire a pore.

Lathyathyathya dzenje malire mayendedwe mbale, oyenera ambiri PCR zida.

Mapepala azoyeserera okhala ndi m'mphepete mwazitsulo amakweza kusindikiza kwa nembanemba ndikuchepetsa chiopsezo chodetsa pakati pa zitsanzo

 

Huida wakhala zaka zambiri ntchito yopanga ndi pokonza mankhwala PCR ndi mfundo makampani mkulu. Mzere wazogulitsa ndiwolemera ndipo ukhoza kukumana ndi mapulogalamu ambiri oyesera a PCR. Bwerani mudzasankhe zogulitsa zoyenera kuyeserera kwanu.

谷歌推广疫情产品PCR


Post nthawi: Jun-03-2021