Ntchito ya Microbial inoculation

Ntchito ya Microbial inoculation

1. Katemera wa Slant (ndi Staphylococcus aureus)
(1) Musanachite opareshoni, pukutani m'manja ndi mowa wa 75%, ndikuyatsa nyali ya mowa mowawo utatha.
(2) Gwirani chubu chovutitsa ndi ndege yokhotakhota pakati pa chala chachikulu cha dzanja lamanzere ndi zala zina zinayi, kuti ndege yokhotakhota ndi mbali yomwe ili ndi zovuta ikhale m'mwamba komanso yopingasa.
(3) Yang'anani kupsyinjika ndi tampon pa ndege yokhazikika poyamba, kuti ikhale yosavuta kutulutsa panthawi ya inoculation.
(4) Gwirani mphete yojambulira m'dzanja lamanzere (monga kunyamula cholembera), ndipo tenthetsani mbali ya mpheteyo ndi lawi lamoto, ndiyeno tenthetsaninso chubu choyesera chomwe chingapitirire mu chubu choyesera.
(5) Gwiritsani ntchito chala cha mphete, chala chaching'ono ndi chikhato cha dzanja lamanja kuti mutulutse chubu chopondereza ndi pulagi ya thonje kapena kapu ya chubu choyesera kuti chilumikizidwe nthawi imodzi, ndiyeno mulole chubu choyesera chilumikizidwe nthawi imodzi. tenthedwa pang'onopang'ono kuti musatenthetse (musatenthe kutentha kwambiri).
(6) Wonjezerani chiwopsezo chowotcha cholowera mu chubu cha bakiteriya, kukhudza kuzungulira kwa khoma lamkati la chubu choyesera kapena sing'anga popanda moss wa bakiteriya, mulole kuti uzizizire, kenako pang'onopang'ono pewani moss wa bakiteriya, ndiyeno chotsani. mabakiteriya ochokera ku mabakiteriya.Chotsani katemera mu chubu cha mbewu.
(7) Wonjezerani mwachangu chipika cha inoculation chodetsedwa ndi kupsyinjika mu chubu china choyezera kuti chilumikizidwe.Kuchokera pansi pa bevel kupita mmwamba, pangani mzere wandiweyani mu mawonekedwe a "Z" mmbuyo ndi mtsogolo.Nthawi zina katemera angagwiritsidwenso ntchito kujambula mzere pakati pa sing'anga kwa katemera katemera, kuti aone kukula kwa kupsyinjika..
(7) Wonjezerani mwachangu chipika cha inoculation chodetsedwa ndi kupsyinjika mu chubu china choyezera kuti chilumikizidwe.Kuchokera pansi pa bevel kupita mmwamba, pangani mzere wandiweyani mu mawonekedwe a "Z" mmbuyo ndi mtsogolo.Nthawi zina katemera angagwiritsidwenso ntchito kujambula mzere pakati pa sing'anga kwa katemera katemera, kuti aone kukula kwa kupsyinjika.
(8) Katemera akatha, tulutsani mphete kuti muwotche pakamwa pa chubu ndikuyiyika ndi pulagi ya thonje.
(9) Yatsani mphete yolowetsamo poiwotcha mofiira.Ikani chipika choyimitsa ndikumangitsa pulagi ya thonje.
2. Katemera wamadzimadzi
(1) Sing'anga yotsetsereka imalumikizidwa ndi sing'anga yamadzimadzi.Njirayi imagwiritsidwa ntchito powona kukula kwa mabakiteriya komanso kutsimikiza kwazomwe zimachitika mthupi.Njira yopangira opaleshoniyi ndi yofanana ndi kale, koma pakamwa pa chubu choyesera amatsamira mmwamba kuti madzi achikhalidwe asatuluke mabakiteriya atayikidwa., pakani mphete ya inoculation ndi khoma lamkati la chubu kangapo kuti mutsuka mabakiteriya pa mphete yapansi.Mukathira, pulagi ya thonje ndikumenya chubu choyesera pang'onopang'ono m'dzanja lamanja kuti abalalitse mabakiteriya.
(2) Ikani sing'anga yamadzimadzi kuchokera mumadzimadzi.Pamene kupsyinjika ndi madzi, ntchito wosabala pipette kapena dropper kwa olowa kuwonjezera pa inoculation loop.Mukakokera, ingotulutsani pulagi ya thonje pafupi ndi lawi lamoto, perekani mphuno pamoto, yamwani madzi a bakiteriya mu njira ya chikhalidwe ndi pipette wosabala, ndikugwedezani bwino.
3. Katemera wa mbale
Tizilombo toyambitsa matenda tinakhala timizeremizere ndipo timawayala m’mbale.
(1) Inoculation ndi mikwingwirima Onani njira yolekana.
(2) Kupaka ndi inoculation Pambuyo kuyamwa njira ya bakiteriya mu mbale ndi wosabala pipette, wogawana kufalitsa pamwamba pa mbale ndi chosawilitsidwa galasi ndodo.
4. Puncture inoculation
Tizilombo toyambitsa matenda timawathira m'malo olimba kwambiri.Njira imeneyi ntchito inoculation wa mabakiteriya anaerobic kapena kuona zokhudza thupi katundu pozindikira mabakiteriya.
(1) Njira yopangira opaleshoni ndi yofanana ndi yomwe ili pamwambapa, koma singano yoyatsira yomwe imagwiritsidwa ntchito iyenera kukhala yowongoka.
(2) Boolani singano ya katemera kuchokera pakati pa sing'anga ya chikhalidwe mpaka itayandikira pansi pa chubu, koma musalowemo, ndipo pang'onopang'ono mutulutse mwa njira yoyamba yoboola.

Microbial inoculation operation


Nthawi yotumiza: Apr-19-2022

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