Momwe mungasankhire zofunikira pa labotale ya PCR (2)

HTiyenera kusankha zogwiritsira ntchito PCR labotale (2)

Kufalikira kwa mliri wa korona watsopano m'malo ena kwakhala chinthu chodetsa nkhawa aliyense. Kudziwika kwa coronavirus yatsopano pakadali pano imagwiritsa ntchito njira yochulukirapo ya PCR yowunikira, ndipo nthawi yomaliza ya lipoti la mayeso a nucleic acid imatenga pafupifupi 4 mpaka 5 maola. Viral nucleic acid ingagwiritsidwe ntchito pozindikira pokhapokha mutachotsa. Musanayese kuyesa zida, m'pofunika kuti mugwire ntchito yomanga makina asanayesedwe pa PCR pamakinawo, kenako ndikusamutsira ku makina a PCR kuti ayambe kuyesa kwa PCR.

Munthawi yonseyi, kuwonjezera madzi ku PCR kumawoneka ngati ntchito yosavuta, koma pakadali pano kuchuluka kwakudziwika kukukulira kwambiri, ndichilumikizano chofunikira kwambiri pantchito. Omwe amagwiritsa ntchito mapampu nthawi zambiri amakhala ndi malingaliro ambiri posankha choyezera choyezera, koma posankha chilinganizo choyezera bomba, samaperekaSamalirani kwambiri izi. Nsonga yoyezera ya pipette ili ngati chipolopolo cha mfuti yoyezera, yomwe imakhudza kwambiri kulondola kwa kupopera, chifukwa chake kufunikira sikutayika. kuyeza bomba la pipette. Little sucker, funsani ku yunivesite, tiyeni tiphunzire limodzi.

PCR 检测 800

1 Ndi ziti zomwe tikufunikira kudziwa kuti tigule nsonga?

"Malingana ngati nsonga yomwe ingayikiridwe ndiye nsonga yomwe ingagwiritsidwe ntchito" - ichi ndi pafupifupi chidziwitso chonse cha ogwiritsa ntchito chosinthira nsonga. Izi zitha kunenedwa kuti ndizowona koma sizowona kwathunthu.

Nsonga yomwe imatha kukhomedwa payipi yoyezera imatha kupangiratu njira yopopera yoyeza kuti muzindikire magwiridwe antchito, koma kodi izi ndi zodalirika? Chizindikiro chofunsa chikufunika apa. Kuyankha funsoli kumafuna kuti deta ilankhule.

(1) Mungafune kuyesa mayeso a ntchito mutayerekezera choyezera choyezera ndi nsonga. Mukatsuka nsonga, yesani zowonjezerapo kangapo, onaninso kuchuluka kwake nthawi iliyonse, ndikulemba kuwerenga.

(2) Terengani kulondola komanso kulondola kwa ntchito yopopera pambuyo poti isinthe kukhala voliyumu kutengera kuchuluka kwa madzi oyeserera.

(3) Zomwe tiyenera kusankha ndi nsonga yolondola komanso yolondola. Ngati choyezera choyezera ndi choyezera chikufanana molondola, zikutanthauza kuti kulimba kwa choyezera choyezera ndi choyezera choyezera sichingatsimikizike, kuti zotsatira za ntchito iliyonse sizingathenso kuberekanso.

 

2 Ndi ziti zomwe tiyenera kudziwa kuti tigule nsonga?

Zizindikiro zogwirira ntchito za nsonga ndi: kutsatsa pang'ono, fyuluta, kulimba, mphamvu yotsitsa ndikuchotsa, palibe DNase ndi RNase, palibe pyrogen; Zizindikirozi zitha kupezeka pazambiri izi.

 

Ubwino wazinthu za 01 Pakadali pano, maupangiri okoka pamsika amapangidwa ndi pulasitiki wa polypropylene (pulasitiki yopanda utoto komanso wowonekera bwino wokhala ndi mankhwala osokoneza bongo komanso kutentha kwakukulu). Malangizo apamwamba nthawi zambiri amapangidwa ndi polypropylene wachilengedwe, pomwe maupangiri otchipa amatha kugwiritsa ntchito pulasitiki ya polypropylene. Nsonga yoperekedwa ndi Huida imapangidwa ndi zinthu zakunja za polypropylene zomwe zimakwaniritsa miyezo ya USP Class-VI. Ili ndi chiwonetsero chokwanira, kukana kwa zosungunulira za organic, komanso kutentha kosiyanasiyana: -80~ 121, palibe mapindikidwe atatha kutentha komanso kuthamanga kwambiri, zopangira zapamwamba Bweretsani magwiridwe antchito!

Kuphatikiza apo, maupangiri ambiri adzawonjezera zowonjezera zowonjezera panthawi yopanga. Ambiri ndi awa:

(1) zida zopangira utoto, zogwiritsa ntchito nsonga zabuluu ndi zachikaso;

(2) zowonjezera. Thandizani nsonga kuti mulekanitsidwe ndi nkhungu mukangopanga.

(3) Zowonjezera zochizira pamwamba pa mfuti yotsatsira otsika kwambiri.

 

02 Kuwononga kwachilengedwe

Ogwiritsa ntchito ochulukirachulukira adzafunika kuti malangizowo akhale opanda RNase, opanda DNase, opanda DNA, opanda pyrogen komanso opanda ATP. Komabe, maupangiri otentha kwambiri sangatsimikizire kuti kulibe tizilombo toyambitsa matenda. Kuonetsetsa kuti palibe kuipitsidwa kwachilengedwe, njira monga chithandizo cha gamma ray zimafunikira. Chifukwa chake, ndizosatheka kuti ogwiritsa ntchito ataye maupangiri wamba kukhala maupangiri osagwirizana ndi biologically, ndipo maupangiri omwe adagulidwa osagwiritsa ntchito zamoyo ayenera kupereka ziphaso zofananira kuti ziwonetse kuchuluka kwa mayeso azinthu zomwe zikugwirizana. Malangizo omwe amaperekedwa ndi Doheng onse amapangidwa mumisonkhano yopanda fumbi ya 100,000 kuti muwonetsetse kuti zopangidwazo zilibe pyrogen, DNase-free, komanso RNase.

 

Gawo la 03Tips ndi mafotokozedwe

Kuphatikiza pa maupangiri omwe amagwiritsidwa ntchito kwambiri, palinso maupangiri a fyuluta, maupangiri otsatsa otsika kwambiri, maupangiri akuntchito, ndi malangizo apakamwa, omwe ali oyenera kuyesa kosiyanasiyana. Mwachitsanzo, nyemba zoyesa kuyeza zopyapyala ndi nsonga ya fyuluta sizingalowe mkatikati mwa bomba loyesera, potero zimateteza mbali zoyesa kuyeza kuchokera ku kuipitsidwa ndi dzimbiri. Chofunika kwambiri, chitha kuwonetsanso kuti sipadzakhala kuipitsidwa kwapakati pa zitsanzo.

Zolemba zake pamunsi pake zimakhala m'matumba ndi mabokosi. Bokosilo limasungunuka asanachoke mufakitole, ndipo wogwiritsa akhoza kuligwiritsa ntchito molunjika; pali maupangiri okwana 500 kapena 1000 thumba limodzi, ndipo ogwiritsa ntchito ambiri adzagula maupangiriwo kenako ndikuyika malangizowo m'bokosi lala. Kupaka m'mabokosi ndiokwera mtengo, koma kulongedza matumba kumawonjezeranso chiopsezo chodetsa pakunyamula pamutu bokosi lazomenyera.

 

Huida ali ndi maupangiri athunthu, onse m'matumba ndi mabokosi. Zolemba zapayipi: 10ul, 100ul, 200ul, 300ul, 1000ul, 1250ul; Palinso maupangiri wamba, maupangiri a fyuluta, ndi maupangiri oyendetsera malo oyenera. . Monga katswiri wothandizila zida zamankhwala & zogwiritsa ntchito, Huida sangangopatsa makasitomala mayankho azachipangizo ndi zida za PCR, ndikuyesetsa kugwiritsa ntchito ma labotale a PCR, titha kupereka zinthu zonse zabwino kwambiri ndi ntchito zina. Mwalandiridwa kutumiza kufunsa kuti mumve zambiri. 

https://www.huidalab.com/pipette-tips/


Post nthawi: Jun-01-2021